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GAT B

Conducted by National Testing Agency (NTA) for DBT and RCB

  1. Computer based test
  2. Institute counselling

Section A at 12th level, Section B in graduate biotechnology, 240 marks in 3 hours

GAT B Syllabus and Exam Pattern Explained

State examsGraduate levelBiotech PG entryUpdated 26 Oct 2026

The GAT B syllabus has two parts: Section A tests physics, chemistry, mathematics and biology at Class 12 level, and Section B tests biotechnology and allied life sciences at bachelor's level. The Graduate Aptitude Test for Biotechnology lasts three hours on screen and offers 160 questions; you respond to 120 of them, and the ceiling is 240 marks.

NTA holds it on behalf of the Department of Biotechnology, while the Regional Centre for Biotechnology frames the syllabus. Institutes backed by DBT fill their biotech M.Sc, M.Tech and M.V.Sc seats from this score. Read on for the marking, each topic group and a sensible weekly split.

Exam at a glance

  • Conducted by

    National Testing Agency, for the Department of Biotechnology
  • Used for

    Admission to DBT supported PG programmes in biotechnology
  • Mode

    Computer based test, English only
  • Questions

    160 in all; attempt 60 in Section A and any 60 of 100 in Section B
  • Maximum marks

    240
  • Time

    3 hours
  • Negative marking

    Minus 0.5 in Section A, minus 1 in Section B
  • Official website

GAT B exam pattern

Marking by section, as in the latest bulletin
SectionLevel and subjectsQuestions to answerCorrectWrongMarks
AClass 12: physics, chemistry, mathematics, biologyAll 60+1Minus 0.560
BBachelor's level biotechnology and life sciencesAny 60 out of 100+3Minus 1180
Total240

Section B carries three quarters of the marks, so it decides your rank. The freedom to skip 40 questions there is your main tool: pick the 60 you are surest of, since each wrong one costs a full mark. In Section A a wrong answer costs half a mark against a gain of one, which makes a guess worthwhile once two choices are out.

Pattern, marks and eligibility are fixed afresh by NTA in each bulletin, so read the newest one on the GAT B and BET portal before planning.

Section A: what the 12th level part covers

This part checks that your science base is sound. Questions come from the Class 11 and 12 courses of the four subjects.

  • Physics: mechanics, heat, waves, electricity and magnetism, optics, modern physics
  • Chemistry: atomic structure, bonding, equilibrium, thermochemistry, basic organic reactions and biomolecules
  • Mathematics: algebra, trigonometry, coordinate geometry, calculus, probability and statistics
  • Biology: cell biology, genetics, physiology, ecology, evolution, biotechnology basics

Students from a pure biology degree often find mathematics and physics rusty. Two weeks with NCERT examples is usually enough to secure easy marks here.

GAT B syllabus for Section B, the graduate part

Section B follows the bachelor's course in biotechnology and allied subjects. These are the ten blocks in the official list.

  • Biomolecules and metabolism: structure of biomolecules, membranes, enzymes, metabolic pathways, bioenergetics
  • Microbiology: viruses, microbial diversity, growth and nutrition, host and pathogen interaction
  • Cell biology: cell organisation, cell cycle, signalling
  • Genetics and molecular biology: replication, transcription, translation, patterns of inheritance, microbial genetics, DNA repair
  • Analytical techniques: microscopy, centrifugation, spectroscopy, chromatography, electrophoresis
  • Immunology: innate and adaptive immunity, antibodies, MHC, hypersensitivity, autoimmunity
  • Bioinformatics: biological databases, sequence alignment, phylogeny, basics of molecular dynamics
  • Recombinant DNA technology: vectors, libraries, cloning, PCR, sequencing, blotting, gene transfer
  • Plant and animal biotechnology: tissue and cell culture, transgenic organisms, hybridomas, stem cells
  • Bioprocess engineering: bioreactors, fermentation kinetics, sterilisation, downstream processing, scale up

Which Section B areas to master first

NTA does not publish a question count per topic. Since you only need 60 of 100, depth in six or seven areas beats a thin layer over all ten.

  • Molecular biology, genetics and recombinant DNA technology sit at the centre of most biotech degrees, so start there.
  • Biochemistry and cell biology link to most other topics and are worth learning thoroughly.
  • Microbiology and immunology suit students from life science or microbiology degrees.
  • Bioprocess engineering and bioinformatics favour B.Tech biotechnology graduates; others can treat them as optional unless time allows.
Farhan has a B.Sc in microbiology and little exposure to bioreactor design. He planned to skip most bioprocess questions and instead built strength in microbiology, immunology, molecular biology and techniques. In his mocks he still found more than 60 questions he could answer with confidence.

A plan spread over ten weeks

  1. Weeks 1 to 2: Section A refresh, one subject at a time, with timed sets.
  2. Weeks 3 to 5: molecular biology, genetics, rDNA technology.
  3. Weeks 6 to 7: biochemistry, cell biology, microbiology, immunology.
  4. Week 8: analytical techniques plus one of bioprocess or bioinformatics.
  5. Weeks 9 to 10: complete mock papers against a three hour clock, rehearsing how you choose your 60 Section B items.

Section B shares much of its content with the CSIR NET life sciences test, useful if a fellowship is also on your list, and the IIT JAM biotechnology syllabus covers similar ground for IIT M.Sc seats.

Who can take GAT B

A bachelor's degree in a relevant field is required; which fields count (biotechnology, life sciences, microbiology, pharmacy, chemistry and others) depends on the programme. Final year students may apply. Recent bulletins fixed the minimum aggregate at 60 percent for the general, OBC NCL and EWS groups, with 55 percent accepted from SC, ST and PwD applicants, with no maximum age for admission. Each participating institute may add its own subject rules, so match your degree with the programme list in the bulletin.

GAT B and BET: not the same paper

NTA holds the Biotechnology Eligibility Test (BET) alongside GAT B, but the two serve different purposes. GAT B leads to postgraduate admission, while BET decides eligibility for the DBT Junior Research Fellowship and has its own syllabus, pattern and age limit. Many candidates register for both. Our entrance exams hub lists more tests open to graduates.

Reading list for both sections

NCERT books cover Section A. For Section B, standard undergraduate texts in biochemistry, molecular biology, microbiology, immunology and genetic engineering are enough; you do not need research level books. The most useful resource is the syllabus and sample material in the official bulletin, which shows the level of questions to expect.

Frequently asked questions

How many questions do I answer in GAT B?

You answer 120 of the 160 questions: all 60 in Section A and any 60 of the 100 in Section B. If you attempt more than 60 in Section B, check the bulletin for which answers are counted.

How much does a wrong GAT B answer cost?

A wrong answer costs 0.5 marks in Section A, where a correct one gains 1, and 1 mark in Section B, where a correct one gains 3. Unattempted questions score zero.

Does GAT B have a maximum age?

Not for PG admission, going by recent bulletins. BET, which is for the fellowship, does have an age limit.

Can a B.Sc chemistry graduate take GAT B?

Eligibility depends on the programme you want. Some programmes accept degrees in chemistry or other sciences, while others need biology or biotechnology. Check the programme wise eligibility table in the bulletin.

Is GAT B held offline?

No. The paper is taken on screen at an allotted test centre, and questions are set only in English.

Does the GAT B syllabus change every year?

The broad areas have been stable, but NTA publishes the syllabus with each bulletin. Compare your notes with the latest one before you begin.